1、生殖與發(fā)育 -- 以海洋經(jīng)濟(jì)無脊椎動(dòng)物為研究對象，從細(xì)胞學(xué)、生理生化和分子生物學(xué)等角度揭示了海葵、單環(huán)刺螠、櫛孔扇貝、鹵蟲、中國明對蝦、南美白對蝦和仿刺參等代表物種的生殖系起源和分化、重要器官的發(fā)生和功能建立；當(dāng)前正在以櫛孔扇貝和單環(huán)刺螠為代表動(dòng)物，開展其性別分化、性腺和體節(jié)發(fā)育以及干細(xì)胞相關(guān)基因與蛋白的結(jié)構(gòu)、表達(dá)和功能研究。

2、硫化物代謝 – 以單環(huán)刺螠為代表動(dòng)物，建立了實(shí)驗(yàn)室和養(yǎng)殖池塘中單環(huán)刺螠代謝硫化物和改良底質(zhì)質(zhì)量的模式；系統(tǒng)闡述了該物種硫化物氧化代謝關(guān)鍵基因的分子特征，揭示了其體內(nèi)兩條線粒體呼吸鏈電子傳遞途經(jīng)；當(dāng)前正在進(jìn)行單環(huán)刺螠硫化物代謝關(guān)鍵基因的轉(zhuǎn)錄調(diào)控、相關(guān)信號(hào)通路在此過程中的作用等研究。

3、細(xì)胞體外培養(yǎng) -- 以櫛孔扇貝為研究對象，突破了貝類細(xì)胞在體外難于傳代的瓶頸，可使扇貝幼蟲細(xì)胞在體外繼代培養(yǎng)19代；目前正在進(jìn)行扇貝等無脊椎動(dòng)物幼蟲和成體細(xì)胞的體外轉(zhuǎn)化和相關(guān)應(yīng)用等研究。

4、動(dòng)物繁育–攻克了單環(huán)刺螠工廠化育苗技術(shù)，現(xiàn)正在開展推廣等應(yīng)用探索。

近年主要研究課題

1、國家自然科學(xué)基金面上項(xiàng)目 “參與單環(huán)刺螠應(yīng)對環(huán)境硫化物的重要信號(hào)通路鑒定和功能分析”，2014-2017，主持；

2、國家自然科學(xué)基金面上項(xiàng)目 “單環(huán)刺螠硫化物氧化代謝的分子特征及硫醌氧化還原酶基因的轉(zhuǎn)錄調(diào)控”，2011-2013，主持；

3、國家自然科學(xué)基金面上項(xiàng)目 “單環(huán)刺螠硫化物代謝關(guān)鍵基因的功能分析”，2008-2010，主持；

4、國家自然科學(xué)基金面上項(xiàng)目 “單環(huán)刺螠不同濃度硫化物環(huán)境中的呼吸代謝機(jī)制”，2006-2006，主持；

5、國家自然科學(xué)基金面上項(xiàng)目 “單環(huán)刺螠早期發(fā)育的研究”，2003-2005，主持；

6、國家十二五863課題 “海洋生物細(xì)胞分子育種關(guān)鍵技術(shù)”， 2012-2015，骨干；

7、國家十一五 863重大項(xiàng)目 “重要海水養(yǎng)殖動(dòng)物細(xì)胞工程育種技術(shù)”，2006-2010，骨干；

8、國家教育部高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金 “vasa基因在蝦貝參生殖系起源、分化中的表達(dá)和功能分析”， 2009-2011，主持；

9、國家自然科學(xué)基金和韓國科技部聯(lián)合資助中韓國際合作項(xiàng)目 “單環(huán)刺螠生殖生物學(xué)”， 2004-2005，中方主持；

10 山東省重點(diǎn)基金 “扇貝性別分化重要基因的鑒定和功能分析”，2008-2011，主持；

11 青島市科技計(jì)劃基礎(chǔ)研究項(xiàng)目 “櫛孔扇貝雌性相關(guān)基因的克隆和功能分析”，2011-2013，主持；

12 青島市海洋漁業(yè)局研發(fā)項(xiàng)目 “單環(huán)刺螠對青島沿海底質(zhì)質(zhì)量改良的研究”，2009-2010，主持。

代表性成果：

已發(fā)表學(xué)術(shù)論文100余篇，其中SCI論文40余篇；申請國家發(fā)明專利5項(xiàng)；參編學(xué)術(shù)專著3部；獲得各類科研獎(jiǎng)勵(lì)4項(xiàng)。

1. Liu Jianguo, Zhifeng Zhang*, Xiaoshi Ma, Shaoshuai Liang, Dandan Yang. Characteristics of 17β-hydroxysteroid dehydrogenase 8 and its potential role in gonads of the Zhikong scallop Chlamys farreri. The Journal of Steroid Biochemistry and Molecular Biology, 2014, 141, 77-86.

2. Jianguo Liu, Zhifeng Zhang*, Litao Zhang, Xiaolong Liu, Dandan Yang, Xiaoshi Ma. Variation of estradiol-17β and testosterone levels correlated with gametogenesis in the gonad of Zhikong scallop (Chlamys farreri) gonad during annual reproductive cycle. Canadian Journal of Zoology, 2014, 92: 195–204.

3. Litao Zhang, Xiaolong Liu, Jianguo Liu, Zhifeng Zhang*. Characteristics and function of sulfur dioxygenase in Echiuran worm Urechis unicinctus. 2013, PLoS ONE 8(12): e81885.

4. Li Yu, Meng Yan, Juan Sui, Wan-Qiang Sheng，Zhi-Feng Zhang*. Expression pattern of vasa gene and gonadogenesis in sea cucumber Apostichopus japonicus during early development. Molecular Reproduction and Development, 2013, 80: 744-752.

5. Jian Huang, Litao Zhang, Jinlong Li, Xiaoli Shi, Zhifeng Zhang*. Proposed function of alternative oxidase in mitochondrial sulfide oxidation detoxification in the Echiuran worm, Urechis unicinctus. Journal of the Marine Biological Association of the United Kingdom. 2013, 93 (8): 2145-2154.

6. MENG YAN, JUAN SUI, WAN-QIANG SHENG, MING-YU SHAO, ZHI-FENG ZHANG*. Expression Pattern of vasa Gene in Gonad of Sea Cucumber Apostichopus japonicus During Gametogenesis and Reproductive Cycle. Gene Expression Patterns, 2013, 13, 171-176.

7. Faiz Muhammad, Zhi-Feng Zhang*, Ming-Yu Shao, Xiao-Li Shi and Muhammad Shafi. Genesis of hematopoietic tissue and its relation with hemocytes of Litopenaeus vannamei (Boone, 1931) (Crustacea: Decapoda). Pakistan Veterinary Journal, 2013, 33 (1): 91-95.

8. Zhenqui Qin, Yun Li, Dapeng Sun, Mingyu Shao, Zhifeng Zhang*. Molecular cloning and expression analysis of the vitellogenin gene in the scallop Chlamys farreri and the effects of estradiol-17<beta>. Invertebrate Biology, 2012, 131(4): 312-321.

9. Yu-Bin Ma, Zhi-Feng Zhang*, Ming-Yu Shao, Kyoung-Ho Kang, Xiao-Li Shi, Li-Tao Zhang. Function of anal sacs and mid-gut in mitochondrial sulfide metabolism from the echiuran worm Urechis unicinctus. Marine Biology Research, 2012, 8, 1026-1031.

10. Yu-Bin Ma, Zhi-Feng Zhang*, Ming-Yu Shao, Kyoung-Ho Kang, Xiao-Li Shi, Li-Tao Zhang. Response of sulfide:quinone oxidoreductase to sulfide exposure in the echiuran worm Urechis unicinctus. Marine Biotechnology, 2012, 14, 245-251.

11. Faiz Muhammad, Zhang Zhi-Feng*, Shao Ming-Yu, Dong Ying-Ping and Muhammad Shafi. Ontogenesis of digestive system in Litopenaeus vannamei (Boone, 1931) (Crustacea: Decapoda). Italian Journal of Zoology, 2012, 79(1): 77–85.

12. Yu-Bin Ma, Zhi-Feng Zhang*, Ming-Yu Shao, Kyoung-Ho Kang, Zhi Tan, Jin-Long Li. Sulfide: quinone oxidoreductase from echiuran worm Urechis unicinctus. Marine Biotechnology, 2011, 13, 93–107.

13. YU Li, SHAO Mingyu, BAO Zhenmin, HU Jingjie, ZHANG Zhifeng*. Effects of environment factors on initiation of sperm motility in sea cucumber Apostichopus japonicus (Selenka). Journal of Ocean University of China, 2011, 10 (2): 165-169.

14. Feng, Zheng-Fu, Zhang, Zhi-Feng*, Shao, Ming-Yu, Zhu, Wei. Developmental expression pattern of the Fc-vasa-like gene, gonadogenesis and development of germ cell in Chinese shrimp, Fenneropenaeus chinensis, Aquaculture, 2011, 314, 202-209.

15. MA Zhuojun, BAO Zhenmin, WANG Sifeng, ZHANG Zhifeng*. Sulfide-based ATP production in Urechis unicinctus. Chinese Journal of Oceanology and Limnology, 2010, 28 (3): 521-526.

16. WANG Sifeng, ZHANG Zhifeng*, CUI He, Kyoung Ho Kang, MA Zhuojun. The tolerance and metabolic adaptation of Urechis unicinctus (Echiura: Urechidae) to sulfide exposure. Journal of Ocean University of China, 2010, 9 (2): 157-161.

17. Qian-Ru Zhou, Zhi-Feng Zhang*, Ming-Yu Shao, Kyoung Ho Kang. Cloning, characterization and expression analysis of the DEAD-box family genes, Fc-vasa and Fc-PL10a, in Chinese shrimp (Fenneropenaeus chinensis). Chinese Journal of Oceanology and Limnology, 2010, 28 (1): 37-45.

18. Zhifeng Zhang*, Sifeng Wang, Kyoung Ho Kang, Mingyu Shao, Zhuojun Ma. Metobolic adaptation of sulfide and its application in Urechis unicinctus. Journal of Biotechnology, 2008, 136S 535.

19. Ming Yu Shao, Zhi Feng Zhang*, Li Yu, Jing Jie Hu, Kyoung Ho Kang. Cyopreservation of sea cucumber Apostichopus japonicus (Selenka) sperm. Aquaculture Research, 2006, 37, 1450-1457.

20. Zhifeng Zhang, Mingyu Shao, Kyoung Ho Kang. Classification of haematopoietic cells and haemocytes in Chinese prawn Fenneropenaeus chinensis. Fish & Shellfish Immunology, 2006, 21,159-169.

21. Zhi Feng Zhang, Ming Yu Shao and Kyoung Ho Kang. Changes of enzyme activity and hematopoiesis in Chinese prawn Fenneropenaeus chinensis (Osbeck) induced by WSSV and zymosan A. Aquaculture Research, 2005, 36 (7): 674-681.

22. Zhifeng Zhang, Mingyu Shao, Kyoung Ho Kang, Zongtao Chen, Jaemin Kim. The study on the cytology and histochemistry of lymphoid organ spheroids in Penaeus chinensis. Aquaculture, 2004, 240, 463-471.

23.張志峰，王華. 一種櫛孔扇貝心臟細(xì)胞體外培養(yǎng)的方法. 國家發(fā)明專利，申請?zhí)枺?01010258209.x

24. 王華，張志峰. 一種誘導(dǎo)扇貝細(xì)胞體外轉(zhuǎn)化的方法. 國家發(fā)明專利，201010258187.7

25. 張志峰，晏萌，季愛昌，畢穎. 一種櫛孔扇貝擔(dān)輪幼蟲細(xì)胞系的培養(yǎng)方法. 國家發(fā)明專利，201210480145.7

26. 晏萌，張志峰，季愛昌，畢穎. 一種櫛孔扇貝擔(dān)輪幼蟲細(xì)胞解離和分離方法. 國家發(fā)明專利，2012105486765

27. 張志峰，晏萌，張立濤，劉建國. 一種單環(huán)刺螠改良水產(chǎn)養(yǎng)殖池塘富硫底質(zhì)的方法. 國家發(fā)明專利，20130512742.8

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